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  • [Construction and identification for cell strain of anti-human seminal plasma phospholipase A2 monoclonal antibody].

[Construction and identification for cell strain of anti-human seminal plasma phospholipase A2 monoclonal antibody].

Zhonghua nan ke xue = National journal of andrology (2002-12-14)
Shu-Kui Wang, Yu-Feng Huang, Xing-Yi Xia, Bao-Tong Li, Zi-Zheng Wang, Meng-Liu Liu
ABSTRACT

To establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb). After having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2 was regarded as an antigen to immune BALB/C mouse to produce anti-human seminal plasma PLA2 McAb. The PLA2 McAb sensitivity and specificity were performed by ELISA technique and Western-blot analysis, respectively. The molecular weight of PLA2 depurated with 245 fold purification from human seminal plasma was about 34,900, while the sensitivity and typing of its McAb were 1:5(6)-1:5(8) and IgM (kappa) with a satisfied Western-Blot results. The PLA2, which had not been reported in international and domestic papers, may be a new type of PLA2. The establish of its McAb will provide significant tools for the research of the relationship between PLA2 in human seminal plasma and male fertility.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DEAE–Sephadex® A-25 chloride form