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  • Enniatin production by the entomopathogenic fungus Verticillium hemipterigenum BCC 1449.

Enniatin production by the entomopathogenic fungus Verticillium hemipterigenum BCC 1449.

The Journal of antibiotics (2005-02-17)
Sumalee Supothina, Masahiko Isaka, Kanyawim Kirtikara, Morakot Tanticharoen, Yodhathai Thebtaranonth
ABSTRACT

Optimal fermentation conditions for enniatin production using the entomopathogenic fungus Verticillium hemipterigenum BCC 1449 have been investigated. Among various liquid media tested, highest efficiency of enniatin production was achieved by fermentation in yeast extract sucrose. Application of this condition to large-scale fermentation resulted in the isolation of three new analogs, O1, O2 and O3, which are closely related isomers that were characterized as an inseparable mixture, along with seven known enniatins.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lithium borohydride, hydrogen-storage grade, ≥90%
Sigma-Aldrich
Lithium borohydride, ≥90%
Sigma-Aldrich
Lithium borohydride, ≥95.0%
Sigma-Aldrich
Lithium borohydride solution, 2.0 M in THF
Sigma-Aldrich
Lithium borohydride solution, 0.5 M in diethyl ether