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  • Incorporation of mitochondrial membrane proteins into liposomes containing acidic phospholipids.

Incorporation of mitochondrial membrane proteins into liposomes containing acidic phospholipids.

The Journal of biological chemistry (1976-11-10)
G D Eytan, M J Matheson, E Racker
PMID185220
ABSTRACT

Cytochrome oxidase, QH2-cytochrome c reductase, and the oligomycin-sensitive adenosine triphosphatase were incorporated into liposomes by a new procedure which yielded unidirectional orientation of the proteins. Cytochrome oxidase was reconstituted in the mitochrondrial orientation and the adenosine triphosphatase in the submitochondrial orientation. Reconstitutions were achieved by incubating the proteins at room temperature with liposomes which contained phosphatidylcholine, phosphatidylethanolamine, and an acidic phospholipid (cardiolipin, phosphatidylinositol, or phosphatidylserine). The incorporation occurred without added detergent or sonication. This incorporation procedure may serve as a model for the insertion of proteins in vivo.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Magnesium chloride hexahydrate, BioUltra, ≥99.0% (KT)