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  • Development of a method for the analysis of seven banned azo-dyes in chilli and hot chilli food samples by pressurised liquid extraction and liquid chromatography with electrospray ionization-tandem mass spectrometry.

Development of a method for the analysis of seven banned azo-dyes in chilli and hot chilli food samples by pressurised liquid extraction and liquid chromatography with electrospray ionization-tandem mass spectrometry.

Talanta (2009-01-29)
Olga Pardo, Vicent Yusà, Nuria León, Agustín Pastor
ABSTRACT

An automated, confirmatory and sensitive procedure has been developed and validated for the determination of Sudan (I-IV), Sudan Orange G, Sudan Red 7B and Para Red in hot chilli food samples. The proposed method includes pressurised liquid extraction (PLE) with acetone, gel permeation chromatography (GPC) clean-up and detection by liquid chromatography (LC) coupled to electrospray ionization in positive mode tandem mass spectrometry (ESI-MS-MS). The main parameters affecting the performance of the different ionization sources and PLE parameters were previously optimised using statistical design of experiments (DOE). The method was in-house validated on chilli powder and chilli meat. Linear calibrations were obtained with correlation coefficients R(2)>0.999. The limits of detection (LOD) and quantification (LOQ) of the method were in the ranges of 0.002-0.012 ng g(-1) and 0.006-0.036 ng g(-1), respectively for chilli powder. The decision limit and detection capability were between 0.005-0.022 ng g(-1) and 0.007-0.026 ng g(-1), respectively for chilli meat. Recoveries ranged from 94% to 105%. The applicability of the method to the determination of azo-dyes in hot chilli products was demonstrated.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sudan Red 7B, for microscopy (Bot., Hist.)
Sigma-Aldrich
Orange G, certified by the Biological Stain Commission, Dye content 80 %
Sigma-Aldrich
Orange G, certified by the Biological Stain Commission
Sigma-Aldrich
Orange G, for NA electrophoresis
Supelco
Sudan IV-d6, analytical standard
Supelco
Sudan Red 7B, analytical standard
Sigma-Aldrich
Sudan Red 7B
Sigma-Aldrich
Sudan II, Dye content 90 %
Supelco
Sudan II, analytical standard