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  • A rapid method with ultra-high-performance liquid chromatography-tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines.

A rapid method with ultra-high-performance liquid chromatography-tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines.

Journal of separation science (2010-06-10)
Zheng Han, Xuesong Liu, Yiping Ren, Lianjun Luan, Yongjiang Wu
ABSTRACT

A speedy and selective ultra-HPLC-MS/MS method for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-ADON, nivalenol and fusarenon X in traditional Chinese medicines (TCMs) was developed. The method was based on one-step sample cleanup using reliable homemade cleanup cartridges. A linear gradient mobile-phase system, consisting of water containing 0.2% aqueous ammonia and acetonitrile/methanol (90:10, v/v) at a flow rate of 0.4 mL/min, and an Acquity UPLC HSS T3 column (100 mm x 2.1 mm, 1.8 microm) were employed to obtain the best resolution of the target analytes. [(13)C(15)]-DON was used as the internal standard to accomplish as accurate as possible quantitation. The established method was further validated by determining the linearity (R(2) > or = 0.9990), sensitivity (LOQ, 0.29-0.99 microg/kg), recovery (88.5-119.5%) and precision (RSD < or = 15.8%). It was shown to be a suitable method for simultaneous determination of DON, 3-ADON, 15-ADON, nivalenol and fusarenon X in various TCM matrices. The utility and practical impact of the method was demonstrated using different TCM samples.

MATERIALS
Product Number
Brand
Product Description

Supelco
Fusarenon X solution, 100 μg/mL in acetonitrile, analytical standard
Sigma-Aldrich
3-Acetyldeoxynivalenol, from Fusarium roseum
Supelco
15-Acetyldeoxynivalenol solution, ~100 μg/mL in acetonitrile, analytical standard
Supelco
3-Acetyldeoxynivalenol
Supelco
3-Acetyldeoxynivalenol solution, ~100 μg/mL in acetonitrile, analytical standard
Sigma-Aldrich
15-O-Acetyl-4-deoxynivalenol from Fusarium graminearum
Supelco
Fusarenon X, analytical standard