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  • Sorbent trapping solid-phase microextraction of fragrance allergens in indoor air.

Sorbent trapping solid-phase microextraction of fragrance allergens in indoor air.

Journal of chromatography. A (2010-07-14)
J Pablo Lamas, Lucia Sanchez-Prado, Marta Lores, Carmen Garcia-Jares, Maria Llompart
ABSTRACT

Exposure to fragrance substances is exponentially increasing in our daily life due to the enhanced use of scented products. Some fragrances are known to be important sensitizers, inhalation being an important exposure pathway in indoor environments. A simple and sensitive method based on solid-phase enrichment and solid-phase microextraction (SPME) followed by gas chromatography-mass spectrometry (GC-MS) has been developed for the analysis of 24 volatile fragrance allergens in indoor air. Suspected allergens present in the air (0.2 m(3)) were adsorbed onto a very small quantity of florisil (25 mg) and then transferred to a SPME fiber in the headspace mode (HS). To the best of our knowledge, this paper describes the first application of SPME for the determination of these compounds in air samples. The experimental parameters affecting the microextraction process have been optimized using a multifactor experimental design strategy. Accuracy, linearity, precision and detection limits (LODs) were evaluated to assess the performance of the proposed method. External calibration, using spiked sorbent standards, and not requiring the complete sampling process (only the SPME step), demonstrated to be suitable for the quantification of all suspected allergens. Recovery studies were performed at three concentration levels (0.04, 1.00 and 50 microg m(-3)), obtaining quantitative recoveries (> or = 85%) in most cases. LOD values at the low ng m(-3) level were achieved for all the target compounds. The application of the method to daily home air samples demonstrated the ubiquity of this kind of fragrance ingredients in quotidian indoor environments, finding 18 of the 24 considered compounds in concentrations ranging from 0.01 to 56 microg m(-3). Benzyl alcohol, linalool, citronellol, ionone and lilial were found in most analyzed samples.

MATERIALS
Product Number
Brand
Product Description

Supelco
SPME Portable Field Sampler, coating PDMS/DVB
Supelco
Florisil® Adsorbent for Chromatography, 30-60 mesh
Supelco
Florisil® Adsorbent for Chromatography, 60-100 mesh
Supelco
Florisil®, <200 mesh, fine powder
Supelco
Florisil®, for the determination of hydrocarbon acc. to ISO 9377-2
Supelco
Florisil® PR, PR grade (analysis acc. to FDA)
Sigma-Aldrich
Florisil®, 100-200 mesh
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with manual holder
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with manual holder
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), fused silica fiber, df 65 μm(PDMS/DVB, for use with manual holder, needle size 23 ga
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with autosampler
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 23 ga, PDMS/DVB StableFlex, for use with autosampler
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga, metal alloy fiber
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with autosampler
Supelco
Florisil®, 100-200 mesh, fine powder
Supelco
Florisil®, PR grade, 60-100 mesh, coarse powder