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  • Membrane-assisted extractive butanol fermentation by Clostridium saccharoperbutylacetonicum N1-4 with 1-dodecanol as the extractant.

Membrane-assisted extractive butanol fermentation by Clostridium saccharoperbutylacetonicum N1-4 with 1-dodecanol as the extractant.

Bioresource technology (2012-05-12)
Shigemitsu Tanaka, Yukihiro Tashiro, Genta Kobayashi, Toru Ikegami, Hideyuki Negishi, Keiji Sakaki
ABSTRACT

A polytetrafluoroethylene (PTFE) membrane was used in membrane-assisted extractive (MAE) fermentation of acetone-butanol-ethanol (ABE) by Clostridium saccharoperbutylacetonicum N1-4. The growth inhibition effects of 1-dodecanol, which has a high partition coefficient for butanol, can be prevented by employing 1-dodecanol as an extractant when using a PTFE membrane. Compared to conventional fermentation, MAE-ABE fermentation with 1-dodecanol decreased butanol inhibition and increased glucose consumption from 59.4 to 86.0 g/L, and total butanol production increased from 16.0 to 20.1g/L. The maximum butanol production rate increased from 0.817 to 0.979 g/L/h. The butanol productivity per membrane area was remarkably high with this system, i.e., 78.6g/L/h/m(2). Therefore, it is expected that this MAE fermentation system can achieve footprint downsizing.

MATERIALS
Product Number
Brand
Product Description

Supelco
1-Dodecanol, Selectophore, ≥98.0%
Sigma-Aldrich
Lauryl alcohol, ≥98%, FG
Sigma-Aldrich
1-Dodecanol, reagent grade, 98%
Sigma-Aldrich
1-Dodecanol, ACS reagent, ≥98.0%