MilliporeSigma
  • Analgesic and antibutyrylcholinestrasic activities of the venom prepared from the Mediterranean jellyfish Pelagia noctiluca (Forsskal, 1775).

Analgesic and antibutyrylcholinestrasic activities of the venom prepared from the Mediterranean jellyfish Pelagia noctiluca (Forsskal, 1775).

Annals of clinical microbiology and antimicrobials (2012-06-14)
Yosra Ayed, Afef Dellai, Hedi Ben Mansour, Hassen Bacha, Salwa Abid
ABSTRACT

Toxins derived from jellyfishes have been exploited as a model for the development of new drug promising applications to treat neurodegenerative diseases. The present work is aimed to evaluate the acute toxicity of crude venom of Pelagia noctiluca and then to screen the analgesic and antibutyrylcholinestrasic (anti-BuChE) activities of the crude venom and its fractions. Sephadex G75 gel was used to separate crude venom of Pelagia noctiluca, which led to some fractions. In addition, in vivo analgesic and in vitro plasma antibutyrylcholinestrasic activities were carried out with Pelagia crude venom and its fractions respectively. The crude venom and its fractions displayed analgesic and anti-BuChE activities at different doses without inducing acute toxicity. Fraction 2 possesses the highest analgesic and antibutyrylcholinestrasic properties. The crude venom and fraction 1 had shown to possess less significant inhibitory activity against analgesic and antibutyrylcholinestrasic models. Based on this study, the crude venom of Pelagia noctiluca is found to be a useful tool for probing pharmacological activity. The purification and the determination of chemical structures of compounds of active fractions of the venom are under investigation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
lysine acetylsalicylic acid, AldrichCPR
Sigma-Aldrich
Sephadex® G-25, Medium
Sigma-Aldrich
Sephadex® G-100, Superfine
Sigma-Aldrich
Sephadex® G-100, BioReagent, for molecular biology, DNA grade, medium
Sigma-Aldrich
Sephadex® G-25, BioReagent, for molecular biology, DNA grade, Superfine
Sigma-Aldrich
Sephadex® G-15, Medium
Sigma-Aldrich
Sephadex® G-25, Fine
Sigma-Aldrich
Sephadex® G-75, Medium
Sigma-Aldrich
Sephadex® G-25, Coarse
Sigma-Aldrich
Sephadex® G-100, Medium
Sigma-Aldrich
Sephadex® G-75, Superfine
Supelco
Sephadex® G-50, Medium
Sigma-Aldrich
Sephadex® G-10, Medium
Sigma-Aldrich
Sephadex® G-50, Fine
Sigma-Aldrich
Sephadex® G-50, Superfine
Sigma-Aldrich
Sephadex® G-50, BioReagent, for molecular biology, DNA grade, medium
Sigma-Aldrich
Sephadex® G-50, BioReagent, for molecular biology, DNA grade, fine