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  • Characterization and prebiotic activity of aqueous extract and indigestible polysaccharide from Anoectochilus formosanus.

Characterization and prebiotic activity of aqueous extract and indigestible polysaccharide from Anoectochilus formosanus.

Journal of agricultural and food chemistry (2012-07-17)
Li-Chan Yang, Wen-Chuan Lin, Ting-Jang Lu
ABSTRACT

Anoectochilus formosanus (Orchidaceae) is a folk medicine in Asia. This study investigated the in vivo and in vitro prebiotic effects of an aqueous extract of A. formosanus (SAEAF) and of an indigestible polysaccharide (AFP) isolated from SAEAF. Chemical analyses showed AFP was mainly composed of arabinogalactan type II (AG-II), with an average molecular weight of 29 kDa. Following 4 weeks of oral administration to rats, SAEAF exhibited prebiotic effects including a decrease in cecum pH and increases of calcium absorption and fecal bifidobacteria. Furthermore, through a bioactivity-guided separation strategy, AFP was proven to be a bifidogenic component in vitro fecal strains fermentation and in vivo administration to mice. In RT-PCR analysis of Bifidobacterium , AFP increased the expression of ABC transporter related to nutrient uptake. Thus, AFP, a polysaccharide from A. formosanus, was demonstrated to be a prebiotic that has a positive health effect on gut microbiota.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(+)-Arabinogalactan, ≥80% (HPLC), from larch wood
Supelco
(+)-Arabinogalactan, for use with Total Dietary Fiber Control Kit, TDF-C10, from larch wood