Skip to Content
MilliporeSigma
  • Phycobiliproteins or C-phycocyanin of Arthrospira (Spirulina) maxima protect against HgCl(2)-caused oxidative stress and renal damage.

Phycobiliproteins or C-phycocyanin of Arthrospira (Spirulina) maxima protect against HgCl(2)-caused oxidative stress and renal damage.

Food chemistry (2012-09-18)
R Rodríguez-Sánchez, R Ortiz-Butrón, V Blas-Valdivia, A Hernández-García, E Cano-Europa
ABSTRACT

Our objective was to determine if the phycobiliproteins of Arthrospira (Spirulina) maxima protect renal cells against mercury-caused oxidative stress and cellular damage in the kidney. We used 40 male mice that were assigned into eight groups: (1) a control group that received 100mM phosphate buffer (PB) ig and 0.9% saline ip, (2) PB+HgCl(2) (5mg/kg ip), (3) PB plus phycobiliproteins (100mg/kg ig), (4) PB plus C-phycocyanin (100mg/kg ig), and four groups receiving HgCl(2)+phycobiliproteins or C-phycocyanin (50, and 100mg/kg ig). The left kidneys were used to determine lipid peroxidation, quantification of reactive oxygen species, and reduced glutathione and oxidised content. The right kidneys were processed for histology. The HgCl(2) caused oxidative stress and cellular damage. All doses of phycobiliproteins or C-phycocyanin prevented enhancement of oxidative markers and they protected against HgCl(2)-caused cellular damage.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
C-Phycocyanin from Spirulina sp., lyophilized powder
Sigma-Aldrich
Mercury(II) chloride, ≥98%
Sigma-Aldrich
Mercury standard solution, suitable for atomic absorption spectrometry, 1 mg/mL Hg, 1000 ppm Hg
Sigma-Aldrich
Mercury(II) chloride, ReagentPlus®, 99%
Sigma-Aldrich
Mercury(II) chloride, ACS reagent, ≥99.5%