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  • Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets.

Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets.

Chemical communications (Cambridge, England) (2012-10-27)
John Crosby, Tom Treadwell, Michelle Hammerton, Konstantinos Vasilakis, Matthew P Crump, David S Williams, Stephen Mann
ABSTRACT

Compartmentalization of the minimal complex of actinorhodin polyketide synthase in coacervate liquid droplets produces enhanced yields of shunt polyketides under conditions of low and high ionic strength.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(diallyldimethylammonium chloride) solution, average Mw 400,000-500,000 (high molecular weight), 20 wt. % in H2O
Sigma-Aldrich
Poly(diallyldimethylammonium chloride) solution, average Mw 200,000-350,000 (medium molecular weight), 20 wt. % in H2O
Sigma-Aldrich
Poly(diallyldimethylammonium chloride) solution, 20 wt. % in H2O
Sigma-Aldrich
Poly(diallyldimethylammonium chloride) solution, average Mw <100,000 (very low molecular weight), 35 wt. % in H2O