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  • Reinvestigation of drugs and chemicals as aquaporin-1 inhibitors using pressure-induced hemolysis in human erythrocytes.

Reinvestigation of drugs and chemicals as aquaporin-1 inhibitors using pressure-induced hemolysis in human erythrocytes.

Biological & pharmaceutical bulletin (2012-11-06)
Takeo Yamaguchi, Yohei Iwata, Shingo Miura, Kazumasa Kawada
ABSTRACT

Recently, we have found that pressure-induced hemolysis is enhanced by inhibiting water transport via aquaporin-1 (AQP1), as seen in p-chloromercuribenzoate (pCMB)-treated erythrocytes. So, using this method we reinvestigated the functions as AQP1 inhibitors of drugs and chemicals such as acetazolamide, sodium nitroprusside, tetraethylammonium ions (TEA(+)), and dimethylsulfoxide (DMSO). The values of hemolysis at 200 MPa were almost unaffected by acetazolamide or sodium nitroprusside, decreased by TEA(+), and increased significantly by DMSO. Furthermore, the erythrocytes were exposed to pCMB in the presence of TEA(+) or DMSO. The enhancement effect of pCMB on pressure-induced hemolysis was unaffected by TEA(+) but attenuated by DMSO. Taken together, these results suggest that, of drugs and chemicals examined here, DMSO only is an AQP1 inhibitor, but the effect of DMSO is small compared with pCMB.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tetraethylammonium iodide, 98%
Sigma-Aldrich
Acetazolamide, ≥99%, powder
Sigma-Aldrich
Tetraethylammonium chloride hydrate
Acetazolamide, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Tetraethylammonium chloride, ≥98% (titration)
Supelco
Tetraethylammonium chloride, for electrochemical analysis, ≥99.0%
Sigma-Aldrich
Tetraethylammonium hydroxide solution, ~25% in methanol (~1.5 M)
Supelco
Tetraethylammonium hydroxide solution, ~1.0 M (CH3CH2)4NOH in H2O, electrochemical grade
Sigma-Aldrich
Tetraethylammonium hydroxide solution, 20 wt. % in H2O
Sigma-Aldrich
Tetraethylammonium bromide, ReagentPlus®, 99%
Sigma-Aldrich
Tetraethylammonium bromide, reagent grade, 98%
Sigma-Aldrich
Tetraethylammonium hydroxide solution, 35 wt. % in H2O
Sigma-Aldrich
Tetraethylammonium chloride, BioUltra, for molecular biology, ≥99.0% (AT)