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  • Protein immobilization on 3C-SiC (100) as a substrate for detecting the onset of acute myocardial infarction (AMI).

Protein immobilization on 3C-SiC (100) as a substrate for detecting the onset of acute myocardial infarction (AMI).

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference (2013-02-01)
A Oliveros, A Guiseppi-Elie, M Jaroszeski, S E Saddow
ABSTRACT

Silicon Carbide (SiC), has been shown to be a bio- and hema-compatible substrate that could potentially be used in biosensor applications. The development of a viable biorecognition interface using SiC as the substrate material for bio-detection is described. Surface modification with 3-aminopropyltriethoxysilane (APTES) and immobilization via covalent conjugation of antimyoglobin (anti-Myo) on the modified surfaces is achieved, which are initial steps for immunosensing based devices. Successful formation of APTES layers and antibody immobilization were identified with surface water contact angle (SWCA), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Silicon carbide, nanopowder, <100 nm particle size
Sigma-Aldrich
Silicon carbide, -200 mesh particle size
Sigma-Aldrich
Silicon carbide, −400 mesh particle size, ≥97.5%