MilliporeSigma

Engineering a 3D vascular network in hydrogel for mimicking a nephron.

Lab on a chip (2013-03-05)
Xuan Mu, Wenfu Zheng, Le Xiao, Wei Zhang, Xingyu Jiang
ABSTRACT

Engineering functional vascular networks in vitro is critical for tissue engineering and a variety of applications. There is still a general lack of straightforward approaches for recapitulating specific structures and functions of vasculature. This report describes a microfluidic method that utilizes fibrillogenesis of collagen and a liquid mold to engineer three-dimensional vascular networks in hydrogel. The well-controlled vascular network demonstrates both mechanical stability for perfusing solutions and biocompatibility for cell adhesion and coverage. This technique enables the mimicry of passive diffusion in a nephron one of the main routes transferring soluble organic molecules. This approach could be used for in vitro modelling of mass transfer-involved physiology in vasculature-rich tissues and organs for regeneration and drug screening.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(ethylene glycol) dimethacrylate, average Mn 6,000, contains 1000 ppm 4-methoxyphenol as inhibitor
Sigma-Aldrich
Poly(ethylene glycol) dimethacrylate, average Mn 20,000, contains MEHQ as inhibitor
Sigma-Aldrich
Poly(ethylene glycol) dimethacrylate, average Mn 10,000, contains MEHQ as inhibitor
Sigma-Aldrich
Poly(ethylene glycol) dimethacrylate, average Mn 750, contains 900-1100 ppm MEHQ as inhibitor
Sigma-Aldrich
Poly(ethylene glycol) dimethacrylate, average Mn 2000, contains ~1000 ppm MeHQ as stabilizer
Sigma-Aldrich
Poly(ethylene glycol) dimethacrylate, average Mn 550, contains 80-120 ppm MEHQ as inhibitor, 270-330 ppm BHT as inhibitor