MilliporeSigma
  • An improved simple colorimetric method for quantitation of non-transferrin-bound iron in serum.

An improved simple colorimetric method for quantitation of non-transferrin-bound iron in serum.

Biochemistry and molecular biology international (1995-03-01)
D Zhang, S Okada, T Kawabata, T Yasuda
ABSTRACT

A simple method for direct quantitation of non-transferrin-bound iron (NTBI) in serum is introduced. NTBI was separated from serum by adding excess nitrilotriacetic acid disodium salt (NTA) to serum to form an Fe-NTA complex and then ultrafiltrated using a micro-filter. The NTBI in the ultrafiltrate was quantitated using a bathophenanthroline-based method. The optimal detection condition and several potential confounding factors were investigated. The actual measurements to samples in vivo and in vitro showed that this method is very practical.

MATERIALS
Product Number
Brand
Product Description

Supelco
Bathophenanthroline, for spectrophotometric det. of Fe in serum, ≥99.0%
Sigma-Aldrich
Bathophenanthroline, 97%