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  • A new selective medium for isolating Listeria spp. from heavily contaminated material.

A new selective medium for isolating Listeria spp. from heavily contaminated material.

Applied and environmental microbiology (1988-01-01)
E S Bannerman, J Bille
ABSTRACT

Food-associated outbreaks of human listeriosis have emphasized the importance and necessity of screening food for the presence of Listeria isolates-selective agar medium combining acriflavine (10 mg/liter) with ceftazidime (50 mg/liter) was developed. A total of 1,099 cheese production specimens were cultured, from which 157 Listeria isolates. (14.3%) grew. When compared with modified McBride agar, the acriflavine-ceftazidime agar recovered more Listeria isolates (98 versus 65%, P less than 0.001) more rapidly (57% after 48 h of incubation of the enrichment broth versus 35%, P less than 0.01) and in greater amounts. Acriflavine-ceftazidime selective agar medium proved to be a highly sensitive medium to recover Listeria spp. from heavily contaminated food products.

MATERIALS
Product Number
Brand
Product Description

Millipore
Columbia Agar, NutriSelect® Plus, suitable for microbiology