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  • Detection of N-monomethyl-lysine generated by metabolic transmethylation.

Detection of N-monomethyl-lysine generated by metabolic transmethylation.

Analytical and bioanalytical chemistry (2005-03-10)
Huba Kalász, Imre Klebovich, Katalin Balogh-Nemes, Agnes Szilágyi, Mária Tihanyi, Tibor Szarvas, József Lengyel
ABSTRACT

Administration of radiolabelled deprenyl to rats resulted in the urinary elimination of a (14)C-labelled N(epsilon)-monomethyl-lysine. An increased level of N(epsilon)-monomethyl-lysine was found following an oral dose of another drug, also containing an N-methyl group. The urine sample was treated with 9-fluorenylmethoxycarbonyl chloride and then subjected to high-performance liquid chromatography (HPLC); the radioactive fraction was identified as N(epsilon)-monomethyl-lysine by using HPLC-MS in electrospray mode. Identification of N(epsilon)-monomethyl-lysine in the radioactive fraction gives experimental proof of transmethylation from a well-known drug to an endogenous compound.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nε-Methyl-L-lysine hydrochloride, ≥98.0% (TLC)