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  • [Biological fixation of nitrogen and growth of bacteria of the genus Azotobacter in liquid media in the presence of perfluorocarbons].

[Biological fixation of nitrogen and growth of bacteria of the genus Azotobacter in liquid media in the presence of perfluorocarbons].

Prikladnaia biokhimiia i mikrobiologiia (2007-10-13)
M K Bakulin, A S Grudtsyna, A Iu Pletneva
ABSTRACT

The addition of perfluorocarbons (perfluorodecalin, carbogal, and perfluoromethyldecalin) to nitrogen-free liquid media during the submerged cultivation of bacteria of the genus Azotobacter was followed by (1) increases in biomass accumulation and nitrogenase activity and (2) fixation of molecular nitrogen. Addition of perfluorodecalin (5 vol %) to the culture medium of A. chroococcum ACB 121 contributed to increases in biomass accumulation, cell concentration (of more than by five times), nitrogenase activity (of 3.4 times), and total nitrogen content in the medium (of 4.5 times).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Perfluoro(methyldecalin), mixture of isomers, technical grade, 80%