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  • Identification of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine by high-performance liquid chromatography.

Identification of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine by high-performance liquid chromatography.

Applied and environmental microbiology (1986-06-01)
R Garcia, W R Hiatt, S Jasinski, P S Sypherd
ABSTRACT

Dansyl derivatives of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine were resolved from other amino acids in proteins by the use of high-performance liquid chromatography. The system was tested with amino acid standard combinations as well as with acid-hydrolyzed proteins known to contain methylated residues. In all cases the methylated lysines were well resolved.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nε-Methyl-L-lysine hydrochloride, ≥98.0% (TLC)