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  • [Assay of 2-phenylbutyric acid in plasma with gas and liquid chromatography].

[Assay of 2-phenylbutyric acid in plasma with gas and liquid chromatography].

Journal of chromatography (1983-10-14)
M T Tsamis, A M Mange, R Farinotti, G Mahuzier
ABSTRACT

Two chromatographic methods which allow the measurement of 2-phenylbutyric acid in serum are described: a gas chromatographic, after silylation, and a reversed-phase high-performance liquid chromatographic. The liquid chromatography with a fluorescent detection, after derivatization by 4-bromomethyl-7-methoxycoumarin, is ten times more sensitive than gas chromatography and 50 ng/ml can be measured in biological liquids.

MATERIALS
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Product Description

Sigma-Aldrich
α-Methylhydrocinnamic acid, 98%