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  • Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum.

Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum.

Colloids and surfaces. B, Biointerfaces (2013-05-15)
Muge Andac, Igor Yu Galaev, Adil Denizli
ABSTRACT

Macroporous cryogels imprinted with human serum albumin (HSA) have been prepared by copolymerization of 2-hydroxyethyl methacrylate with a functional co-monomer of N-methacryloyl-L-phenylalanine. The cryogels were used for the depletion of HSA from human serum. HSA-imprinted cryogels were prepared with gel fraction yields up to 90%, and their chemical structure, morphology and porosity were characterized by FTIR-spectroscopy, scanning electron microscopy, swelling studies and flow dynamics. Selective binding experiments were performed in the presence of competitive proteins like human transferrin and myoglobin. Albumin-imprinted cryogel column was optimized for fast protein liquid chromatography. Sodium-dodecyl sulfate polyacrylamide gel electrophoresis was used to show the efficiency of albumin depletion.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(2-hydroxyethyl methacrylate), BioReagent, powder, suitable for cell culture
Sigma-Aldrich
Poly(2-hydroxyethyl methacrylate), average Mv 20,000
Sigma-Aldrich
Poly(2-hydroxyethyl methacrylate), average Mv 300,000, crystalline
Sigma-Aldrich
Poly(2-hydroxyethyl methacrylate), average Mv 1,000,000