Surface modification of mammalian cells with stimuli-responsive polymers.

Surface modification of mammalian cells with stimuli-responsive polymers.

Chemical communications (Cambridge, England) (2013-07-26)

Yasuhiko Iwasaki, Mizuki Sakiyama, Shuetsu Fujii, Shin-ichi Yusa

ABSTRACT

In order to introduce alternative functions into mammalian cells and control them under ambient conditions, poly(N-isopropyl acrylamide) (PNIPAM) was immobilized on the cell surface. Cellular aggregation could be regulated by temperature change. In addition, separation of PNIPAM-conjugated glycoproteins was successfully performed.

MATERIALS

Product Number

Brand

Product Description

724459

Sigma-Aldrich

Poly(N-isopropylacrylamide), carboxylic acid terminated, average M_n 10,000

535311

Sigma-Aldrich

Poly(N-isopropylacrylamide), M_n ~40,000

900188

Sigma-Aldrich

Poly(N-isopropyl acrylamide), NHS ester end functionalized, average M_n 5,000