MilliporeSigma
  • Effect of cadmium on the physiological parameters and the subcellular cadmium localization in the potato (Solanum tuberosum L.).

Effect of cadmium on the physiological parameters and the subcellular cadmium localization in the potato (Solanum tuberosum L.).

Ecotoxicology and environmental safety (2013-08-14)
Dongyu Xu, Zhifan Chen, Ke Sun, Dong Yan, Mingjie Kang, Ye Zhao
ABSTRACT

The pollution of agricultural soils with cadmium (Cd) has become a serious problem worldwide. The potato (Solanum tuberosum L.) was used to investigate how different concentrations of Cd (1, 5, and 25mgkg(-1)) affected the physiological parameters and the subcellular distribution of Cd in the potato. The analyses were conducted using scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX). The results suggest that the leaf is the organ with the highest accumulation of Cd. The malondialdehyde (MDA) content increased and the chlorophyll content decreased in response to high level of Cd. The SEM-EDX microanalysis revealed that Cd was primarily deposited in the spongy and palisade tissues of the leaf. Furthermore, Cd was also detected in the cortex and the adjacent phloem and was observed inside the intercellular space, the interior surface of the plasma membrane, and on the surface of the elliptical starch granules in the tubers of the potato. Although low concentrations of Cd migrated from the root to the tuber, the accumulation of Cd in the tuber exceeded the standard for food security. Therefore, the planting of potato plants in farmland containing Cd should be seriously evaluated because Cd-containing potatoes might present high health risk to humans.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Starch, puriss. p.a., from potato, reag. ISO, reag. Ph. Eur., soluble
Sigma-Aldrich
Starch from potato, Powder
Sigma-Aldrich
Starch from corn, practical grade
Sigma-Aldrich
Starch from corn, Unmodified waxy corn starch of essentially pure amylopectin; contains only trace amounts of amylose.
Sigma-Aldrich
Starch from potato, suitable for electrophoresis
Sigma-Aldrich
Starch from wheat, Unmodified
Sigma-Aldrich
Starch from rice
Sigma-Aldrich
Starch from potato, Soluble
Sigma-Aldrich
Starch, from potato, tested according to Ph. Eur.
Supelco
Starch from corn, for use with Total Dietary Fiber Control Kit, TDF-C10
Supelco
Starch from corn, analytical standard, analytical standard for Starch Assay Kits SA-20 and STA-20
Sigma-Aldrich
Starch from corn