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  • Evaluation of antimicrobial activity of silver nanoparticles for carboxymethylcellulose film applications in food packaging.

Evaluation of antimicrobial activity of silver nanoparticles for carboxymethylcellulose film applications in food packaging.

Journal of nanoscience and nanotechnology (2014-04-25)
Maria C Siqueira, Gustavo F Coelho, Márcia R de Moura, Joana D Bresolin, Silviane Z Hubinger, José M Marconcini, Luiz H C Mattoso
ABSTRACT

In this study, silver nanoparticles were prepared and incorporated into carboxymethylcellulose films to evaluate the antimicrobial activity for food packaging applications. The techniques carried out for material characterization were: infrared spectroscopy and thermal analysis for the silver nanoparticles and films, as well as particle size distribution for the nanoparticles and water vapor permeability for the films. The antimicrobial activity of silver nanoparticles prepared by casting method was investigated. The minimum inhibitory concentration (MIC) value of the silver nanoparticles to test Gram-positive (Enterococcus faecalis) and Gram-negative (Escherichia coli) microorganisms was carried out by the serial dilution technique, tested in triplicate to confirm the concentration used. The results were developed using the Mcfarland scale which indicates that the presence or absence of turbidity tube demonstrates the inhibition of bacteria in relation to the substance inoculated. It was found that the silver nanoparticles inhibited the growth of the tested microorganisms. The carboxymethylcellulose film embedded with silver nanoparticles showed the best antimicrobial effect against Gram-positive (E. faecalis) and Gram-negative (E. coli) bacteria (0.1 microg cm(-3)).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium carboxymethyl cellulose, average Mw ~250,000, degree of substitution 1.2
Sigma-Aldrich
Sodium carboxymethyl cellulose, viscosity 50-200 cP , c=4% H2O at 25­°C
Sigma-Aldrich
Sodium carboxymethyl cellulose, average Mw ~250,000, degree of substitution 0.9
Sigma-Aldrich
Sodium carboxymethyl cellulose, average Mw ~250,000, degree of substitution 0.7
Sigma-Aldrich
Silver, colloidal, 65-75% Ag basis
Sigma-Aldrich
Silver, foil, thickness 1.0 mm, 99.99% trace metals basis
Sigma-Aldrich
Sodium carboxymethyl cellulose, average Mw ~700,000
Sigma-Aldrich
Carboxymethylcellulose sodium, meets USP testing specifications, Medium viscosity
Sigma-Aldrich
Silver nanowires, diam. × L 200  nm × 10 μm, 0.5% (isopropyl alcohol suspension)
Sigma-Aldrich
Silver, conductive paste
Silver, foil, light tested, 50x50mm, thickness 0.0125mm, 99.95+%
Silver, foil, not light tested, 100x100mm, thickness 0.0025mm, 99.97%
Silver, foil, not light tested, 100x100mm, thickness 0.0125mm, 99.95+%
Silver, foil, 6mm disks, thickness 0.015mm, 99.97%
Silver, foil, 6mm disks, thickness 0.125mm, annealed, 99.95+%
Silver, foil, 8mm disks, thickness 0.007mm, 99.97%
Silver, foil, 8mm disks, thickness 0.035mm, as rolled, 99.95+%
Silver, foil, light tested, 100x100mm, thickness 0.025mm, as rolled, 99.99+%
Silver, foil, light tested, 150x150mm, thickness 0.05mm, annealed, 99.95+%
Silver, foil, light tested, 50x50mm, thickness 0.015mm, 99.97%
Silver, foil, 8mm disks, thickness 0.25mm, as rolled, 99.95+%
Silver, foil, 8mm disks, thickness 0.008mm, 99.95+%
Silver, foil, not light tested, 100x100mm, thickness 0.003mm, 99.9%
Silver, foil, 6mm disks, thickness 0.5mm, as rolled, 99.99+%
Silver, foil, light tested, 150x150mm, thickness 0.05mm, as rolled, 99.95+%
Silver, foil, not light tested, 150x150mm, thickness 0.025mm, as rolled, 99.95+%
Silver, foil, 8mm disks, thickness 1.0mm, as rolled, 99.95+%
Silver, foil, 6mm disks, thickness 0.5mm, as rolled, 99.95+%
Silver, foil, 6mm disks, thickness 1.0mm, as rolled, 99.95+%
Silver, foil, light tested, 150x150mm, thickness 0.025mm, as rolled, 99.95+%