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NCAM and lung cancer.

International journal of cancer. Supplement = Journal international du cancer. Supplement (1994-01-01)
R Michalides, B Kwa, D Springall, N van Zandwijk, J Koopman, J Hilkens, W Mooi
ABSTRACT

The presence of the neural cell adhesion molecule, NCAM, is indicative for a poor prognosis in lung-cancer patients. Using MAb 735, we have investigated the expression of polysialic acid, PSA, on NCAM in a spectrum of neuro-endocrine lung tumors, ranging from the slowly growing typical carcinoids via the atypical carcinoids with clinically unpredictable behavior to the highly aggressive small-cell lung carcinomas. Our immunohistochemical findings indicate a significant association between the presence of PSA on the tumor cells and an aggressive and immature sub-type of the tumor. This might be related to impairment of cell-cell and cell-matrix interactions by the presence of PSA, as we demonstrated in vitro, since detachment is one of the first steps in the metastatic process. The NCAM-MAb 123C3 used in these studies appeared extremely useful in immunoscintigraphy and immunotherapy of SCLC xenografts in nude mice, and for immunoscintigraphy of a SCLC patient. This may be explained by internalization of the 123C3 antibody, which we demonstrated in vitro. 123C3 is the only Cluster-I SCLC MAb studied thus far that becomes internalized.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
CD56 (123C3.D5) Mouse Monoclonal Antibody
Sigma-Aldrich
CD56 (MRQ-42) Rabbit Monoclonal Antibody