MilliporeSigma
  • Effect of time between adhesive application and photoactivation on adhesion and collagen exposure.

Effect of time between adhesive application and photoactivation on adhesion and collagen exposure.

American journal of dentistry (2015-02-25)
Débora Lopes Salles Scheffel, Renata Pereira Estrêla, Paula Maciel Pires, Matheus Racy Mariusso, Carlos Alberto de Souza Costa, Josimeri Hebling
ABSTRACT

To evaluate the influence of the time elapsed between the application and photoactivation of Single Bond 2 (SB) on microtensile bond strength (μTBS) and collagen exposure at the adhesive interface produced in the presence of intrapulp pressure. Dentin occlusal surfaces were prepared in 72 molars, and divided into eight groups (n = 9). After acid etching, SB was applied, with or without simulated intrapulp pressure, and remained undisturbed for 0, 20, 40 or 60 seconds, before photoactivation. Three teeth/group were processed for staining with Goldner trichrome and evaluation of the thickness of exposed collagen zone (CZ) at the base of the hybrid layer. Composite resin build-ups were placed on the remaining six prepared teeth prior to sectioning for microtensile testing. Data were submitted to ANOVA and Tukey tests (α = 0.05). In the absence of pressure, immediate photoactivation resulted in the lowest μTBS, while the other groups did not differ among them. Under intrapulp pressure, the lowest values were observed after 60 seconds. There was no difference in the thickness of the exposed collagen zone among the groups without pressure. However, thicker layers were recorded in the presence of pressure after 40 and 60 seconds. Waiting 60 seconds between application and photoactivation of SB significantly reduced resin-dentin bond strength when pulpal pressure was simulated.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Collagen from bovine nasal septum, Bornstein and Traub Type II, powder
Sigma-Aldrich
Collagen from calf skin, Bornstein and Traub Type I, (0.1% solution in 0.1 M acetic acid), aseptically processed, BioReagent, suitable for cell culture
Sigma-Aldrich
Collagen from calf skin, Bornstein and Traub Type I, solid, BioReagent, suitable for cell culture
Sigma-Aldrich
Collagen from bovine achilles tendon, powder, suitable for substrate for collagenase
Sigma-Aldrich
Collagen from rabbit skin, Bornstein and Traub Type I, powder
Sigma-Aldrich
Collagen Type IV from human cell culture, Bornstein and Traub Type IV, 0.3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Collagen, Type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type IV, powder
Sigma-Aldrich
Collagen human, Bornstein and Traub Type I, acid soluble, powder, ~95% (SDS-PAGE)
Sigma-Aldrich
Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type IV, powder
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type I (Sigma Type VIII), powder
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type III (Sigma Type X), powder
Sigma-Aldrich
Collagen from rat tail, Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Collagen from bovine tracheal cartilage, Bornstein and Traub Type II, powder
Sigma-Aldrich
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type IV, solution, suitable for cell culture, High Performance