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  • Emulsive polymerization as a method of enzyme modification preserving biological properties of nanostructures.

Emulsive polymerization as a method of enzyme modification preserving biological properties of nanostructures.

Bulletin of experimental biology and medicine (2012-01-12)
I P Gontar, G F Sycheva, A V Alexandrov, L N Shilova, E S Simakova, N N Emelyanov, N N Matasova, L A Maslakova, A B Zborovsky
ABSTRACT

The proposed method of emulsive polymerization provides the possibility of modifying and obtaining insoluble forms of superoxide dismutase (SOD), glutathione reductase, and streptolysin-O preserving nanoobjects (conformationally active centers and antigenic determinants) in their native states. Apart from enzymatic and immunological properties, the samples acquired some new features: resistance to high temperature, resistance to 3 M KCNS solution and buffer solutions with high concentration of hydrogen ions, and resistance to preserving solutions. Magnetic properties provide the possibility of simplifying enzyme-linked immunosorbent and immunofluorescence assays. In addition, sensitivity of these assays was by an order of magnitude higher and the specificity fully preserved. Taking all the facts into account, we prepared agents for long-term and repeated use. Due to preserved enzymatic properties, insoluble forms of SOD and glutathione reductase can be considered as a tool for correction of peroxide-antioxidant balance and associated immunological abnormalities.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glutathione Reductase from baker's yeast (S. cerevisiae), ammonium sulfate suspension, 100-300 units/mg protein (biuret)