MilliporeSigma
  • Development of dispersive liquid-liquid microextraction technique using ternary solvents mixture followed by heating for the rapid and sensitive analysis of phthalate esters and di(2-ethylhexyl) adipate.

Development of dispersive liquid-liquid microextraction technique using ternary solvents mixture followed by heating for the rapid and sensitive analysis of phthalate esters and di(2-ethylhexyl) adipate.

Journal of chromatography. A (2015-01-04)
Mir Ali Farajzadeh, Leila Khoshmaram
ABSTRACT

In this study, for the first time, a dispersive liquid-liquid microextraction technique using a ternary solvent mixture is reported. In order to extract five phthalate esters and di(2-ethylhexyl) adipate with different polarities from aqueous samples, a simplex centroid experimental design method was used to select an optimal mixture of ternary solvents prior to gas chromatographyflame ionization detection. In this work, dimethyl formamide as a disperser solvent containing dichloromethane, chloroform, and carbon tetrachloride as a ternary extraction solvent mixture is injected into sample solution and a cloudy solution is formed. After centrifuging, 250μL of the obtained sedimented phase was transferred into another tube and 5μL DMF was added to it. Then, the tube was heated in a water bath at 75°C for 5min in order to evaporate the main portion of the extraction solvents. Finally, 2μL of the remained phase is injected into the separation system. Under the optimum extraction conditions, the method shows wide linear ranges and low limits of detection and quantification between 0.03-0.15 and 0.09-0.55μgL(-1), respectively. Enrichment factors and extraction recoveries are in the ranges of 980-4500 and 20-90%, respectively. The method is successfully applied in the determination of the target analytes in mineral water, soda, lemon juice, vinegar, dough, and yogurt packed in plastic packages.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Diisobutyl phthalate, 99%
Sigma-Aldrich
Carbon tetrachloride, anhydrous, ≥99.5%
Supelco
Carbon tetrachloride, analytical standard
Sigma-Aldrich
Dibutyl phthalate, 99%
Sigma-Aldrich
Dimethyl phthalate, ≥99%
Supelco
Bis(2-ethylhexyl) phthalate, Selectophore
Sigma-Aldrich
Dimethyl phthalate, ≥99%
Supelco
Density Standard 1191 kg/m3, H&D Fitzgerald Ltd. Quality
Sigma-Aldrich
Diethyl pyrocarbonate, 96% (NT)
Supelco
Diisobutyl phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Bis(2-ethylhexyl) phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
Dibutyl phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Plastic additive 01, European Pharmacopoeia (EP) Reference Standard
Dibutyl phthalate, European Pharmacopoeia (EP) Reference Standard
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetone, Laboratory Reagent, ≥99.5%
Sigma-Aldrich
Carbon tetrachloride, reagent grade, 99.9%
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)
Sigma-Aldrich
Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Supelco
Carbon tetrachloride, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Acetone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.8%
Sigma-Aldrich
Acetone, histological grade, ≥99.5%
Supelco
Dibutyl phthalate, PESTANAL®, analytical standard
Supelco
Bis(2-ethylhexyl) phthalate, PESTANAL®, analytical standard
Supelco
Dimethyl phthalate, PESTANAL®, analytical standard