Skip to Content
MilliporeSigma
  • Activation of HIFa pathway in mature osteoblasts disrupts the integrity of the osteocyte/canalicular network.

Activation of HIFa pathway in mature osteoblasts disrupts the integrity of the osteocyte/canalicular network.

PloS one (2015-03-26)
Gui-lai Zuo, Lian-fang Zhang, Jin Qi, Hui Kang, Peng Jia, Hao Chen, Xing Shen, Lei Guo, Han-bing Zhou, Jin-shen Wang, Qi Zhou, Nian-dong Qian, Lian-fu Deng
PMID25806791
ABSTRACT

The hypoxia-inducible factors (HIFs), HIF-1α and HIF-2α, are the central mediators of the homeostatic response that enables cells to survive and differentiate in low-oxygen conditions. Previous studies indicated that disruption of the von Hippel-Lindau gene (Vhl) coincides with the activation of HIFα signaling. Here we show that inactivation of Vhl in mature osteoblasts/osteocytes induces their apoptosis and disrupts the cell/canalicular network. VHL-deficient (ΔVHL) mice exhibited a significantly increased cortical bone area resulting from enhanced proliferation and osteogenic differentiation of the bone marrow stromal cells (BMSCs) by inducing the expression of β-catenin in the BMSC. Our data suggest that the VHL/HIFα pathway in mature osteoblasts/osteocytes plays a critical role in the bone cell/canalicular network and that the changes of osteocyte morphology/function and cell/canalicular network may unleash the bone formation, The underlying mechanism of which was the accumulation of β-catenin in the osteoblasts/osteoprogenitors of the bone marrow.

MATERIALS
Product Number
Brand
Product Description
13-1920
Sigma-Aldrich
Hydrogen peroxide solution, SAJ first grade, ≥30.0%
12-0995
Sigma-Aldrich
Glutaraldehyde solution, SAJ first grade, 20.0-26.0%
G5882
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
G7651
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
G7776
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
H1009
Sigma-Aldrich
Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
G6257
Sigma-Aldrich
Glutaraldehyde solution, Grade II, 25% in H2O
95294
Sigma-Aldrich
Hydrogen peroxide solution, tested according to Ph. Eur.
21049
Supelco
Calcium standard for AAS, analytical standard, 1.000 g/L Ca+2 in hydrochloric acid, traceable to BAM
49629
Sigma-Aldrich
Glutaraldehyde solution, technical, ~50% in H2O (5.6 M)
88597
Millipore
Hydrogen peroxide solution, 3%, suitable for microbiology
W512303
Sigma-Aldrich
Glutaric dialdehyde solution, 50 wt. % in H2O, FCC
316989
Sigma-Aldrich
Hydrogen peroxide solution, contains potassium stannate as inhibitor, 30-32 wt. % in water, semiconductor grade, 99.999% trace metals basis
340855
Sigma-Aldrich
Glutaraldehyde solution, 50 wt. % in H2O
95321
Supelco
Hydrogen peroxide solution, ≥30%, for trace analysis
18304
Sigma-Aldrich
Hydrogen peroxide solution, 34.5-36.5%
323381
Sigma-Aldrich
Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2O
516813
Sigma-Aldrich
Hydrogen peroxide solution, 50 wt. % in H2O, stabilized
31642
Sigma-Aldrich
Hydrogen Peroxide Solution, 30% (w/w), puriss. p.a., reag. ISO, reag. Ph. Eur.
H3410
Sigma-Aldrich
Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H2O, meets USP testing specifications
216763
Sigma-Aldrich
Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H2O, ACS reagent
95299
Sigma-Aldrich
Hydrogen peroxide solution, purum p.a., ≥35% (RT)
349887
Sigma-Aldrich
Hydrogen peroxide solution, contains inhibitor, 35 wt. % in H2O
16911
Supelco
Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis
G6403
Sigma-Aldrich
Glutaraldehyde solution, 50% in H2O, suitable for photographic applications
G7526
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
05-0535
Sigma-Aldrich
Calcium chloride solution, 0.025 M
09-1320
Sigma-Aldrich
Ethylenediaminetetraacetic acid, SAJ special grade, ≥99.0%
431788
Sigma-Aldrich
Ethylenediaminetetraacetic acid, 99.995% trace metals basis
21115
Sigma-Aldrich
Calcium chloride solution, BioUltra, for molecular biology, ~1 M in H2O