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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetone, suitable for chromatography, ≥99.8%
Sigma-Aldrich
Chloroform, suitable for HPLC
Sigma-Aldrich
Acetone, suitable for residue analysis, JIS 5000
Sigma-Aldrich
Tetrahydrofuran, JIS special grade, ≥99.5%
Sigma-Aldrich
Diethyl ether, suitable for residue analysis, JIS 5000
Sigma-Aldrich
Tetrahydrofuran, suitable for HPLC, contains no stabilizer
Sigma-Aldrich
Methanol, SAJ special grade
Sigma-Aldrich
Tetrahydrofuran, SAJ first grade, ≥99.0%
Sigma-Aldrich
1-Octanol, JIS special grade, ≥98.0%
Sigma-Aldrich
N,N-Dimethylformamide, JIS special grade, ≥99.5%
Sigma-Aldrich
Formaldehyde solution, 10%
Sigma-Aldrich
Sodium chloride, JIS special grade, ≥99.5%
Sigma-Aldrich
1-Octanol, SAJ first grade, ≥75.0%
Sigma-Aldrich
Methanol, JIS special grade, ≥99.8%
Sigma-Aldrich
Acetone, suitable for residue analysis, ≥99.5%
Sigma-Aldrich
Sodium chloride, SAJ first grade, ≥99.0%
Sigma-Aldrich
Diethyl ether, JIS special grade, ≥99.5%
Sigma-Aldrich
Acetone, JIS special grade, ≥99.5%
Sigma-Aldrich
Diethyl ether, JIS 1000, suitable for residue analysis, ≥99.5%
Sigma-Aldrich
Diethyl ether, SAJ first grade, ≥99.0%
Sigma-Aldrich
Acetone, SAJ first grade, ≥99.0%
Sigma-Aldrich
Sodium chloride solution, 1 M
Sigma-Aldrich
2-Mercaptoethanol, SAJ special grade, ≥99.0%
Sigma-Aldrich
Diethyl ether, ≥99.5%
Sigma-Aldrich
Sodium chloride solution, 0.1 M
Sigma-Aldrich
N,N-Dimethylformamide, SAJ first grade, ≥99.0%
Sigma-Aldrich
Formaldehyde solution, JIS special grade, 36.0-38.0%, contains methanol as stabilizer
Sigma-Aldrich
Piperidine, SAJ first grade, ≥99.0%
Sigma-Aldrich
Methanol, JIS 300, ≥99.8%, suitable for residue analysis
Sigma-Aldrich
Chloroform, JIS 300, ≥99.0%