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  • Simultaneous determination of 11 major components in Palmul-tang by HPLC-DAD and LC-MS-MS.

Simultaneous determination of 11 major components in Palmul-tang by HPLC-DAD and LC-MS-MS.

Journal of chromatographic science (2013-06-07)
Bohyoung Lee, Jin Bae Weon, Bo-Ra Yun, Jiwoo Lee, Min Rye Eom, Choong Je Ma
ABSTRACT

Palmul-tang, a traditional herbal medicine, is composed of eight herbs (Ginseng radix, Glycyrrhizae radix, Hoelen, Atractylodis rhizoma, Angelicae gigantis radix, Cnidii rhizoma, Paeoniae radix and Rehmanniae radix) and exhibits various bioactivities, including antiallergic and antitumor effects. In this study, an effective, reliable and accurate high-performance liquid chromatography method has been developed for the simultaneous determination of 11 marker components in Palmul-tang: hydroxymethylfurfural, albiflorin, paeoniflorin, ferulic acid, nodakenin, ginsenoside Rg1, decursinol, glycyrrhizin, 6-gingerol, ginsenoside Rg3 and decursin. All calibration curves of the 11 components indicated excellent linearity (correlation coefficient > 0.9997) within the test range. The limits of detection and quantification of each component were in the ranges of 0.08-1.03 and 0.23-3.11 µg/mL, respectively. The intra-day and inter-day relative standard deviation values were within 1.65 and 2.71%, respectively. The mean recovery values were 94.49 to 101.10%. The established method was successfully applied to the simultaneous determination of 11 major components in 12 commercial samples of Palmul-tang. The developed analytical method is simple and suitable for the quality control of Palmul-tang.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
[6]-Gingerol, ≥98% (HPLC)
Supelco
Ginsenoside Rg1, analytical standard
Ginsenoside Rg1, primary reference standard
Supelco
[6]-Gingerol, analytical standard
Sigma-Aldrich
Paeoniflorin, ≥98% (HPLC)
Sigma-Aldrich
Albiflorin, ≥95% (LC/MS-ELSD)
Ferulic acid, European Pharmacopoeia (EP) Reference Standard
Supelco
Paeoniflorin, analytical standard
Supelco
Ginsenoside Rg3, analytical standard
Sigma-Aldrich
trans-Ferulic acid, 99%
Sigma-Aldrich
trans-Ferulic acid, ≥99%
Supelco
trans-Ferulic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)
Supelco
trans-Ferulic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Ginsenoside Rg3, ≥98% (HPLC)