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  • High-Throughput Continuous Flow Production of Nanoscale Liposomes by Microfluidic Vertical Flow Focusing.

High-Throughput Continuous Flow Production of Nanoscale Liposomes by Microfluidic Vertical Flow Focusing.

Small (Weinheim an der Bergstrasse, Germany) (2015-09-24)
Renee R Hood, Don L DeVoe
ABSTRACT

Liposomes represent a leading class of nanoparticles for drug delivery. While a variety of techniques for liposome synthesis have been reported that take advantage of microfluidic flow elements to achieve precise control over the size and polydispersity of nanoscale liposomes, with important implications for nanomedicine applications, these methods suffer from extremely limited throughput, making them impractical for large-scale nanoparticle synthesis. High aspect ratio microfluidic vertical flow focusing is investigated here as a new approach to overcoming the throughput limits of established microfluidic nanoparticle synthesis techniques. Here the vertical flow focusing technique is utilized to generate populations of small, unilamellar, and nearly monodisperse liposomal nanoparticles with exceptionally high production rates and remarkable sample homogeneity. By leveraging this platform, liposomes with modal diameters ranging from 80 to 200 nm are prepared at production rates as high as 1.6 mg min(-1) in a simple flow-through process.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(dimethylsiloxane), viscosity 1.0 cSt (25 °C)
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Decahydronaphthalene, mixture of cis + trans, reagent grade, 98%
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Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
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Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer
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Decahydronaphthalene, mixture of cis + trans, anhydrous, ≥99%
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Cholesterol, Plant-Derived, SyntheChol®
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SyntheChol® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, sterile-filtered, aqueous solution, suitable for cell culture
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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
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Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
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