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  • DNA synthesis and epigenetic modification during mouse oocyte fertilization by human or hamster sperm injection.

DNA synthesis and epigenetic modification during mouse oocyte fertilization by human or hamster sperm injection.

Journal of assisted reproduction and genetics (2010-11-26)
Yong-Nan Xu, Xiang-Shun Cui, Jin-Cheol Tae, Yong-Xun Jin, Nam-Hyung Kim
ABSTRACT

To evaluate DNA synthesis and epigenetic modification in mouse oocytes during the first cell cycle following the injection of human or hamster sperm. Mouse oocytes following the injection of human and hamster sperm and cultured in M16 medium. Male and female pronucleus formation, DNA synthesis, histone protein modification, and heterochromatin formation were similar in mouse oocytes injected with human or hamster sperm. However, DNA methylation patterns were altered in mouse oocytes following human sperm injection. Immunocytochemical staining with a histone H3-MeK9 antibody revealed that human and hamster sperm chromatin associated normally with female mouse chromatin, then entered into the metaphase and formed normal, two-cell stage embryos. Although differences in epigenetic modification of DNA were observed, fertilization and cleavage occurred in a species non-specific manner in mouse oocytes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9, ascites fluid, clone 1MOD-1A9, Chemicon®
Sigma-Aldrich
Anti-5-Methylcytosine Mouse mAb (162 33 D3), liquid, clone 162 33 D3, Calbiochem®