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  • Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis.

Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis.

Reproduction (Cambridge, England) (2016-12-07)
Feng Wang, Zheng Chen, Xiaofang Ren, Ye Tian, Fucheng Wang, Chao Liu, Pengcheng Jin, Zongyue Li, Feixiong Zhang, Baochang Zhu
ABSTRACT

Hormone-sensitive lipase-knockout (HSL-/-) mice exhibit azoospermia for unclear reasons. To explore the basis of sterility, we performed the following three experiments. First, HSL protein distribution in the testis was determined. Next, transcriptome analyses were performed on the testes of three experimental groups. Finally, the fatty acid and cholesterol levels in the testes with three different genotypes studied were determined. We found that the HSL protein was present from spermatocyte cells to mature sperm acrosomes in wild-type (HSL+/+) testes. Spermiogenesis ceased at the elongation phase of HSL-/- testes. Transcriptome analysis indicated that genes involved in lipid metabolism, cell membrane, reproduction and inflammation-related processes were disordered in HSL-/- testes. The cholesterol content was significantly higher in HSL-/- than that in HSL+/+ testis. Therefore, gene expression and cholesterol ester content differed in HSL-/- testes compared to other testes, which may explain the sterility of male HSL-/- mice.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bouin′s solution, histological fixative
Supelco
F.A.M.E. Mix GLC-10, certified reference material, pkg of 100 mg (Neat)
Supelco
Fatty Acid Kit, (individually packaged, quantities indicated), analytical standard