MilliporeSigma
  • Wnt/β-catenin promotes gastric fundus specification in mice and humans.

Wnt/β-catenin promotes gastric fundus specification in mice and humans.

Nature (2017-01-05)
Kyle W McCracken, Eitaro Aihara, Baptiste Martin, Calyn M Crawford, Taylor Broda, Julie Treguier, Xinghao Zhang, John M Shannon, Marshall H Montrose, James M Wells
ABSTRACT

Despite the global prevalence of gastric disease, there are few adequate models in which to study the fundus epithelium of the human stomach. We differentiated human pluripotent stem cells (hPSCs) into gastric organoids containing fundic epithelium by first identifying and then recapitulating key events in embryonic fundus development. We found that disruption of Wnt/β-catenin signalling in mouse embryos led to conversion of fundic to antral epithelium, and that β-catenin activation in hPSC-derived foregut progenitors promoted the development of human fundic-type gastric organoids (hFGOs). We then used hFGOs to identify temporally distinct roles for multiple signalling pathways in epithelial morphogenesis and differentiation of fundic cell types, including chief cells and functional parietal cells. hFGOs are a powerful model for studying the development of the human fundus and the molecular bases of human gastric physiology and pathophysiology, and also represent a new platform for drug discovery.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-BHLHA15 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-CLDN18 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-GIF antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Retinoic acid, ≥98% (HPLC), powder