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  • Schwann cells promote post-traumatic nerve inflammation and neuropathic pain through MHC class II.

Schwann cells promote post-traumatic nerve inflammation and neuropathic pain through MHC class II.

Scientific reports (2017-10-04)
Maike Hartlehnert, Angelika Derksen, Tim Hagenacker, David Kindermann, Maria Schäfers, Mathias Pawlak, Bernd C Kieseier, Gerd Meyer Zu Horste
ABSTRACT

The activation of T helper cells requires antigens to be exposed on the surface of antigen presenting cells (APCs) via MHC class II (MHC-II) molecules. Expression of MHC-II is generally limited to professional APCs, but other cell types can express MHC-II under inflammatory conditions. However, the importance of these conditional APCs is unknown. We and others have previously shown that Schwann cells are potentially conditional APCs, but the functional relevance of MHC-II expression by Schwann cells has not been studied in vivo. Here, we conditionally deleted the MHC-II β-chain from myelinating Schwann cells in mice and investigated how this influenced post-traumatic intraneural inflammation and neuropathic pain using the chronic constriction injury (CCI) model. We demonstrate that deletion of MHC-II in myelinating Schwann cells reduces thermal hyperalgesia and, to a lesser extent, also diminishes mechanical allodynia in CCI in female mice. This was accompanied by a reduction of intraneural CD4+ T cells and greater preservation of preferentially large-caliber axons. Activation of T helper cells by MHC-II on Schwann cells thus promotes post-traumatic axonal loss and neuropathic pain. Hence, we provide experimental evidence that Schwann cells gain antigen-presenting function in vivo and modulate local immune responses and diseases in the peripheral nerves.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-S100 A1, N-Terminal antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-Neurofilament L Antibody, serum, Chemicon®