GC Analysis of Fragrance Compounds in Shampoo Base on a 100% Methyl Column after SPME using 100 μm PDMS Fiber

SPME fiber

polydimethylsiloxane, 100 μm film (57300-U)

extraction

headspace, 5 min, 80 °C (with sample stirring)

desorption process

5 min, 250 °C

desorption temp.

-50 °C w/liquid CO₂

column

100% methyl polysiloxane, 50 m × 0.25 mm I.D., 0.25 μm (Supelco equivalent, Equity-1, 28047-U)

oven

35 °C to 240 °C at 3 °C/min

inj. temp.

split, 250 °C

detector

MSD, scan m/z = 29-350 AMU

carrier gas

helium, 0.5 mL/min

sample

0.5 g shampoo base with 1% fragrance test mix, diluted 1:1 with NaCl/water in a 10 mL vial