MilliporeSigma

GC Analysis of Residual Solvents in Hemp Extract on Supel-Q PLOT after Headspace SPME using 75 μm Carboxen®/PDMS Fiber

GC Analysis of Residual Solvents in Hemp Extract on Supel-Q™ PLOT after Headspace SPME using 75 μm Carboxen®/PDMS Fiber suitable for GC, application for SPME

Materials

Analytical column

Product No.
Description
Pricing

Supel-Q PLOT Capillary GC Column

L × I.D. 30 m × 0.32 mm, average thickness 15 μm

SPME fiber

Product No.
Description
Pricing

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS)

df 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler

accessory

Product No.
Description
Pricing

Inlet Liner, Direct (SPME) Type, Straight Design (unpacked)

pkg of 1 ea

Headspace vial, screw top, rounded bottom (vial only)

volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 ea

Magnetic Screw Cap for Headspace Vials, 18 mm thread

PTFE/silicone septum (white PTFE/blue silicone), septum thickness 1.5 mm, pkg of 100 ea

Molded Thermogreen® LB-2 Septa, with injection hole

diam. 11 mm, pkg of 50 ea

CONDITIONS

sample preparation

Solid Phase Microextraction

sample/matrix

5 g sample in 10 mL vial

SPME fiber

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS) (57319)

extraction

5 min, headspace, 40 °C

desorption process

3 min, 320 °C; split 10:1

sample preparation

fiber post bake: 2 min, 320 °C

column

Supel-Q PLOT, 30 m x 0.32 mm I.D. (24242)

oven

50 °C (5 min), 10 °C/min to 230 °C (5 min)

inj. temp.

320 °C

detector

MSD

MSD interface

260 °C

scan range

m/z 29-200

carrier gas

helium, 2 mL/min constant flow

injection

split, 10:1

liner

SPME, 0.75 mm I.D.

Description

Analysis Note

Marijuana oil is produced by extraction of the flower buds of the cannabis sativa plant. This process often involves the use of solvents such as hexane or some alcohols. Even though measures are taken to remove these solvents after extraction, some residual material may remain behind in the final product. In this work, headspace SPME was used to analyze residual solvents from an extract of hemp. For the analysis, hemp extract was used for testing in lieu of marijuana extract, which could not be obtained in the location where the work was done. Hemp and marijuana are both varieties of cannabis sativa, however hemp is a variety that is very low in THC content.

Legal Information

Carboxen is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel-Q is a trademark of Sigma-Aldrich Co. LLC