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GC Analysis of Volatiles in Limoncello on SLB®-5ms after SPME using a 100 μm PDMS Fiber

GC Analysis of Volatiles in Limoncello on SLB®-5ms after SPME using a 100 μm PDMS Fiber application for SPME, suitable for GC

Materials

analytical column

Product No.
Description
Pricing

SLB®-5ms Capillary GC Column

L × I.D. 30 m × 0.25 mm, df 0.25 μm

SPME fiber

Product No.
Description
Pricing

SPME fiber assembly Polydimethylsiloxane (PDMS)

df 100 μm(PDMS, needle size 24 ga, for use with manual holder

related product

Product No.
Description
Pricing

SPME Fiber Holder

for use with manual sampling

SPME Fiber Holder

for use with CTC CombiPAL and Gerstel MPS2

standard

Product No.
Description
Pricing

Decanal

≥98% (GC), liquid

3,7-Dimethyl-2,6-octadienyl acetate

mixture of isomers, >97.0% (sum of isomers, GC)

(R)-(+)-Limonene

analytical standard

p-Cymene

analytical standard

α-Terpinene

≥95.0% (GC)

γ-Terpinene

analytical standard

Neryl acetate

analytical standard

(+)-α-Pinene

analytical standard

Myrcene

analytical standard

Geraniol

analytical standard

Nonyl acetate

≥97%, FCC

Valencene

natural, ≥65%

Terpinolene

≥95%, FG

Camphene

analytical standard

Nonanal

analytical standard

Linalool

analytical standard

Citral

analytical standard

(−)-α-Bisabolol

analytical standard

Octyl acetate

analytical standard

α-Terpineol

analytical standard

Octanal

analytical standard

α-Phellandrene

natural, ≥85%, FG

Citronellyl acetate

analytical standard

Undecanal

analytical standard

α-Humulene

analytical standard

CONDITIONS

sample/matrix

Limoncello diluted 1:100 in water, then 5 mL of this solution transferred to a 10 mL crimped vial spiked with 100 μL of a 100 ppm solution of nonane (internal standard)

SPME fiber

100 μm polydimethylsiloxane (57300-U)

extraction

headspace, with a pre-equilibration period of 10 min. at 40 °C, and then a fiber exposure of 30 min. at 40 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 ppm

desorption process

250 °C for 5 min

column

SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)

oven

40 °C, 3 °C/min to 250 °C (2 min)

inj. temp.

250 °C

detector

FID, 260 °C (sampling rate 80 ms)

carrier gas

helium, 35.0 cm/sec

injection

splitless

liner

0.75 mm I.D. SPME (2633901)

sample

Limoncello liquor

Description

Analysis Note

In this application, SPME was used for the analysis of volatiles in limoncello using a headspace method. SPME is ideal for the analysis of aroma volatiles in flavored beverages such as liquors. A 100 μm PDMS fiber was inserted into the headspace of the limoncello sample for 30 minutes at 40 °C followed by GC analysis using FID detection. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.

Legal Information

SLB is a registered trademark of Merck KGaA, Darmstadt, Germany