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HPLC Analysis of Sugars on Ascentis® Si

HPLC Analysis of Sugars on Ascentis® Si application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

Maltotriose

≥90% (HPLC)

Sucrose

BioUltra, for molecular biology, ≥99.5% (HPLC)

Lactose Monohydrate

analytical standard

Glucose

European Pharmacopoeia (EP) Reference Standard

Xylose

European Pharmacopoeia (EP) Reference Standard

Maltose monohydrate

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

Description

Analysis Note

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany