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GC Analysis of Organophosphorus Pesticides (OP-Pest) in Cabbage, Onion, and Mushrooms on SLB-5ms after SPE using Supelclean ENVI-Carb II/PSA

GC Analysis of Organophosphorus Pesticides (OP-Pest) in Cabbage, Onion, and Mushrooms on SLB-5ms after SPE using Supelclean™ ENVI-Carb™ II/PSA application for SPE, suitable for GC

Materials

analytical column

Product No.
Description
Pricing

SLB®-5ms Capillary GC Column

L × I.D. 30 m × 0.25 mm, df 0.25 μm

SPE tube or plate

Product No.
Description
Pricing

Supelclean ENVI-Carb II/PSA SPE Tube

bed B 500 mg (Supelclean PSA SPE), bed A 500 mg (Supeclean ENVI-Carb-II SPE), volume 6 mL, pk of 30

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

10 g homogenized food sample (green onions, apples, Napa cabbage, button mushrooms)

SPE tube/cartridge

Supelclean ENVI-Carb II/PSA SPE Tube (54067-U)

condition

5 mL acetone:toluene (65:35)

sample addition

1 mL sample extract

elution

10 mL of acetone:toluene (65:35)

eluate post-treatment

Evaporate fraction to 0.5 mL then adjust volume to 1 mL with ethyl acetate for GC-NPD analysis

column

SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)

oven

110 °C (2 min), 2.5 °C/min to 205 °C, 10 °C/min to 310 °C (10 min)

inj. temp.

250 °C

detector

NPD, 310 °C

carrier gas

helium, 0.9 mL/min constant flow

injection

2 μL, splitless

liner

4 mm I.D. dual tapered

Description

Preparation Note

Sample pretreatment: To a 10 g sample of homogenized food (green onions, apples, Napa cabbage, button mushrooms) was added 10 mL acetonitrile and 4 g magnesium sulfate (that had been heated at 550 °C) and 1 g sodium chloride. After centrifugation, the supernatant was removed and mixed with 1 g magnesium sulfate and centrifuged. 5 mL of the supernatant was removed and evaporated to 1 mL for SPE loading.

Analysis Note

Organophosphorous pesticide use in agriculture is widespread due to the fact that they are more amenable to environmental degradation in comparison to organochlorine or organonitrogen compounds. There are a large number of pesticides in the organophosphorous group, and because of their potential health effects, they are of particular concern on produce imported from areas in which they are commonly used. This chromatogram illustrates the separation of 63 of these pesticides by GC on the SLB-5ms using nitrogen phosphorus detection (NPD).

Legal Information

ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC