MilliporeSigma

HPLC Analysis of Atrazine Herbicides on Ascentis® Express C18

HPLC Analysis of Atrazine Herbicides on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

standard

Product No.
Description
Pricing

Atrazine

PESTANAL®, analytical standard

Isoproturon

PESTANAL®, analytical standard

Linuron

PESTANAL®, analytical standard

Diuron

PESTANAL®, analytical standard

Metazachlor

PESTANAL®, analytical standard

Methabenzthiazuron

PESTANAL®, analytical standard

Metobromuron

PESTANAL®, analytical standard

Metoxuron

PESTANAL®, analytical standard

Terbuthylazine-2-hydroxy

PESTANAL®, analytical standard

Monolinuron

PESTANAL®, analytical standard

Cyanazine

PESTANAL®, analytical standard

Hexazinone

PESTANAL®, analytical standard

CONDITIONS

column

Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] 20 mM ammonium acetate, pH 6.4 unadjusted; [B] acetonitrile

gradient

20 to 28% B in 11 min; 28 to 65% B in 5 min; held at 65% B for 4 min

flow rate

0.6 mL/min

column temp.

46 °C

detector

UV, 240 nm

injection

5 μL

sample

10-25 μg/mL in 10:90, water: acetonitrile

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 17 triazine pesticides.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany