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HPLC Analysis of Cysteine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)

HPLC Analysis of Cysteine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

guard cartridge

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Guard Column

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

standard

Product No.
Description
Pricing

L-Cysteine

from non-animal source, BioReagent, suitable for cell culture, ≥98%

L-Cysteine

L-Cysteine

BioUltra, ≥98.5% (RT)

D-Cysteine

≥99% (RT)

DL-Cysteine

technical grade

L-Cysteine

≥97%, FG

L-Cysteine

97%

L-Cysteine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Astec CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (40:60:0.02, A:B:C)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

(200 μg /mL in 50:50 water:methanol)

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC