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HPLC Analysis of Organic Acids on Ascentis® RP-Amide

HPLC Analysis of Organic Acids on Ascentis® RP-Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® RP-Amide HPLC Column

3 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

Citric acid

99%

Fumaric acid

≥99.0% (T)

L-(+)-Tartaric acid

≥99.5%

Acrylic acid

anhydrous, contains 200 ppm MEHQ as inhibitor, 99%

Lactic acid

Pharmaceutical Secondary Standard; Certified Reference Material

CONDITIONS

column

Ascentis RP-Amide, 10 cm x 2.1 mm I.D., 3 μm particles (565301-U)

mobile phase

[A] 0.1% (v/v) TFA in [B] water

flow rate

0.3 mL/min

column temp.

35 °C

detector

UV, 210 nm

injection

1 μL

sample

0.2 - 4 g/L in mobile phase

Description

Analysis Note

This application demonstrates the suitability of Ascentis RP-Amide for the analysis of organic acids under 100% aqueous conditions.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany