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HPLC Analysis of PAHs in Canola Oil on SUPELCOSIL LC-PAH after SPE using Supelclean EZ-POP NP

HPLC Analysis of PAHs in Canola Oil on SUPELCOSIL™ LC-PAH after SPE using Supelclean™ EZ-POP NP application for SPE, application for HPLC

Materials

analytical column

Product No.
Description
Pricing

SUPELCOSIL LC-PAH HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

SPE tube or plate

Product No.
Description
Pricing

Supelclean EZ-POP NP

volume 12 mL, pkg of 20

standard

Product No.
Description
Pricing

PAH Calibration Mix

TraceCERT®, certified reference material, 10 μg/mL each component in acetonitrile

Polynuclear Aromatic Hydrocarbons Mix

certified reference material, 2000 μg/mL each component in benzene: dichloromethane (50:50), ampule of 1 mL

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

Canola oil spiked with PAHs at 10 ng/g

SPE tube/cartridge

Supelclean EZ-POP NP, 12 mL, pk of 20 (54341-U)

condition

10 mL acetone, dry under 10-15" mercury for 10 minutes

sample addition

0.5 mL weighed accurately onto cartridge

elution

15 mL acetonitrile

eluate post-treatment

concentrate to final volume of 0.5mL at 40 °C under nitrogen

column

SUPELCOSIL LC-PAH 25 cm x 4.6 mm I.D., 5 μm particles (58229)

mobile phase

[A] water [B] acetonitrile

gradient

40% B held for 5 min; to 100% B in 15 min; held at 100% B for 12 min

flow rate

2.4 mL/min

pressure

2790 psi at start (192 bar)

column temp.

25 °C

detector

Fluorescence, programmed.

Description

Analysis Note

PAHs are ubiquitous in the environment, resulting from both natural and manmade sources of combustion. They can contaminate food crops through environmental exposure to particulates, automobile exhaust, etc. In the case of edible oils, additional PAHs can be introduced during actual production processes such as seed drying [1]. In this application, EZ-POP NP SPE is used to extract PAHs from soybean oil. The 16 PAHs designated in many US EPA methods (less acenaphthylene) were analyzed by HPLC-FLD after extraction from a spiked sample of oil.

Other Notes

Detector: Naphthalene, Acenaphthene, Fluorene - 225nm/320nm; Phenanthrene, Anthracene - 250nm/368nm; Fluoranthene, Pyrene - 237nm/440nm; Benzo[a]anthracene, Chrysene - 265nm/380nm; Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[a]pyrene, Dibenzo[a,h]anthracene - 280nm/420nm; Benzo[g,h,i]perylene, Indeno[1,2,3-cd]pyrene, Benzo[b]chrysene - 300nm/466nm

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC