HPLC Analysis of Polar and Non-Polar Analytes on Ascentis^® Express C8, Column Test Mixture

column

Ascentis Express C8, 5 cm × 4.6 mm I.D., 2.7 μm particles (58336-U)

mobile phase

[A] water; [B] acetonitrile; (45:55, A:B)

flow rate

1.76 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

5 μL