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HPLC Analysis of Polar Neutral and Acidic Amino Acids on Ascentis® Si (Silica)

HPLC Analysis of Polar Neutral and Acidic Amino Acids on Ascentis® Si (Silica) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Si, 10 cm × 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0, with formic acid; [B] water, [C] acetonitrile

gradient

5% A constant; 10% B, 85% C to 16% B, 79% C in 20 min

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI (+), full scan

injection

2 μL

sample

100 μg/mL in 10:90, (50 mM ammonium formate / formic acid, pH 3.0):acetonitrile

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany