HPLC Analysis of Polar Neutral and Acidic Amino Acids on Ascentis^® Si (Silica)

column

Ascentis Si, 10 cm × 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0, with formic acid; [B] water, [C] acetonitrile

gradient

5% A constant; 10% B, 85% C to 16% B, 79% C in 20 min

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI (+), full scan

injection

2 μL

sample

100 μg/mL in 10:90, (50 mM ammonium formate / formic acid, pH 3.0):acetonitrile