HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems
Materials
Analytical column
Product No.
Description
Pricing
Zenix® SEC-300 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steelmobile phase component
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
CONDITIONS
column
Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)
mobile phase
[A] 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid; [B] 0.1 M ammonium bicarbonate (pH unadjusted)
flow rate
0.3 mL/min
column temp.
25 °C
detector
UV, 280 nm
detector
1000 psi
injection
2.5 μL
sample
in mobile phase
Description
Analysis Note
This application compares selectivity of proteins when chromotographed by size exclusion with different buffer systems.
Legal Information
Zenix is a registered trademark of Sepax Technologies