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HPLC Analysis of Silymarin on Ascentis® Express C18

HPLC Analysis of Silymarin on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

standard

Product No.
Description
Pricing

Taxifolin

analytical standard

Silybin

mixture of Silybin A and B, primary reference standard

Isosilybin B

analytical standard

Isosilybin A

analytical standard

Silychristin

analytical standard

Silydianin

analytical standard

CONDITIONS

column

Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] water with 0.1% formic acid; [B] methanol

gradient

held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min

flow rate

0.6 mL/min

pressure

4105 psi (283 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

20 μL

sample

100 μg/mL in 90:10, water:methanol

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of silymarin.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany