HPLC Analysis of Steroidal Glycosides on Ascentis^® Phenyl

column

Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)

mobile phase

[A] 10 mM ammonium formate (pH 3 with formic acid); [B] methanol

flow rate

1.25 mL/min

column temp.

35 °C

detector

ELSD at 45 °C, gain 6, 3.5 bar N2

injection

20 μL

sample

as indicated in mobile phase A